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The Zero Maze is a circular behavioral test apparatus used to assess anxiety-related behavior in rats and mice. It consists of an elevated circular track with two opposing open sections and two opposing enclosed sections. Animals are placed on the maze, and their movements between open and enclosed areas are monitored. Time spent in the open sections versus the enclosed sections indicates levels of anxiety, with more time in the open sections suggesting lower anxiety. The Zero Maze is a valuable tool in neuroscience research for studying the effects of drugs, genetics, and environmental factors on anxiety.